Composite
Part:BBa_K2751014:Design
Designed by: Ming-Sheng Hsu Group: iGEM18_NYMU-Taipei (2018-10-09)
ALB-mEGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 780
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mEGFP reporter protein is fused to the ALB signal peptide.
Source
mEGFP is mutation product of EGFP, which is amplified by conducting PCR on plasmids provided by Dr. Chung-Chih Lin of NYMU.
ALB is amplified by PCR on HEK293 genome.